Combination of multiple isomorphous replacement and anomalous dispersion data for protein structure determination. II. Correlation of the heavy-atom positions in different isomorphous protein crystals
نویسندگان
چکیده
منابع مشابه
HAD, a data bank of heavy-atom binding sites in protein crystals: a resource for use in multiple isomorphous replacement and anomalous scattering.
Information on the preparation and characterization of heavy-atom derivatives of protein crystals has been collected, either from the literature or directly from protein crystallographers, and assembled in the form of a heavy-atom data bank (HAD). The data bank contains coordinate data for the heavy-atom positions in a form that is compatible with the crystallographic data in the Brookhaven Pro...
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Substantial highly correlated differences sometimes exist between a series of heavy-atom derivatives of a macromolecule and the native structure. Use of such a series of derivatives for phase determination by multiple isomorphous replacement (MIR) has been difficult because MIR analysis has treated errors as independent. A simple Bayesian approach has been used to derive probability distributio...
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The structure determination of Sindbis virus core protein using isomorphous replacement and molecular replacement averaging between two crystal forms.
The structure of Sindbis virus core protein has been determined by a combination of multiple isomorphous replacement and molecular replacement averaging techniques. The multiple isomorphous replacement phase determinations were made for two crystal forms (P2(1) and P4(3)2(1)2) of the core protein. The real-space molecular replacement averaging was subsequently carried out between two copies of ...
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A direct-methods procedure has been proposed for separating the phase doublet resulting from the use of either isomorphous replacement or anomalous scattering techniques. The phase doublet is expressed as ~#u= ~h±lA~.l. Formulae combining the structure-factor relationships with the phase-doublet information are given. Problems concerning the practical applications are also discussed. A test cal...
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ژورنال
عنوان ژورنال: Acta Crystallographica
سال: 1965
ISSN: 0365-110X
DOI: 10.1107/s0365110x6500172x